Reference #: 1009158
SHIFT:Day (United States of America)
Seeking Breakthrough Makers
Children's Hospital of Philadelphia (CHOP) offers countless ways to change lives. Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.
At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families. Join us as we build on our promise to advance pediatric care-and your career.
CHOP's Commitment to Diversity, Equity, and Inclusion
CHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace. We are a team dedicated to fostering an environment that allows for all to be their authentic selves. We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.
We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.
A Brief Overview
The Weber Lab at The Children's Hospital of Philadelphia and The University of Pennsylvania Perelman School of Medicine seeks an experienced research assistant to help develop approaches to enhance chimeric antigen receptor (CAR-T) cell therapeutics.
CAR-T cell therapies have revolutionized the cancer immunotherapy landscape by demonstrating remarkable clinical responses in patients with B cell malignancies. However, T cell exhaustion and poor persistence limit efficacy in patients and are major barriers to progress for the treatment of solid tumors. The Weber Lab is developing a suite of approaches (ex. overexpression of engineered proteins, CRISPR-mediated genetic deletion, and small molecule treatment) to transcriptionally and epigenetically reprogram T cells to enhance anti-tumor potency, while simultaneously uncovering molecular mechanisms that govern human T cell fate and function.
The candidate will design and perform pre-clinical studies that includes maintenance of human T cell and tumor cell cultures, T cell engineering (ex. viral production and T cell transduction), cloning and molecular assays, in vitro killing assays to interrogate CAR-T cell anti-tumor function, multi-color flow cytometry, and in vivo experimentation. In addition to designing and performing experiments, the candidate will also be expected to analyze, interpret, and present the data, as well as train new members of the lab.
tocols with minimal direction and guidance from the principal investigator, program director or senior scientists. May exercise moderate discretion and judgment in the execution and interpretation of experiments, and of data derived.This role participates in planned research projects by performing research pro
What you will do
Performs research procedures on experimental samples with minimal supervision
Formulates and performs complex lab experiments and analyzes/ reports the data
Performs tasks that require knowledge of advanced techniques and lab equipment
Performs advanced calculations and maintains appropriate log and lab notebooks
Assists with planning specific research protocols and procedures
Assists in development & troubleshooting of new techniques and ideas
May be required to modify scientific methods and protocols (in conjunction with PI)
Checks others' results for completeness and accuracy
May be responsible for training and mentoring new staff
May assist in the preparation of grants journal articles, posters and oral presentations
May perform routine and non-routine tests / experiments as needed
Maintains office, lab area, reagents, supplies and equipment
Performs routine maintenance on lab equipment
Coordinates schedules of tests & procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Conducts literary searches as required
Education Qualifications
High School Diploma / GED Required
Bachelor's Degree Preferred
Experience Qualifications
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Required
At least three (3) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Preferred
Skills and Abilities
Advanced understanding of lab procedures, protocols, and equipment.
Intermediate proficiency with lab specific experiments / techniques.
Intermediate scientific analysis skills.
Excellent verbal and written communications skills
Excellent organizational skills
Strong critical thinking / problem-solving skills
Strong analytical skills
To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine.
Employees may request exemptions for valid religious and medical reasons. Start dates may be delayed until candidates are immunized or exemption requests are reviewed.
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Children's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.
