You can find the cures you know are out there. At UCLA Health, you make it happen as part of a groundbreaking Research team, working to understand hundreds of medical disorders and improving processes for dozens of procedures across the globe.
Under the direction of the PI, the incumbent is responsible for molecular biology aspects of the research project. Duties include carrying out molecular biology procedures, i.e. cloning, mutagenesis, sequencing, polymerase chain reaction, preparation of recombinant plasmids, and general recombinant DNA methods. Duties also include culturing cells and carrying out biochemical assays. The incumbent will be engaged in research projects involving cloning and characterization of genes. The incumbent will maintain an inventory of cDNA, DNA plasmid stocks, the lab and equipment in order.
Required:
Knowledge of molecular biology techniques
Skills and extensive experience in human and mouse cell culture technique,
Skills in cryo-section
Skills in isolating and purifying high quality DNA and RNA
Skill in sub-cloning of cell lines
Ability to use molecular biology software
Ability and experience to perform immunohistochemistry
Knowledge of cell culture, including thawing cells, culturing cells and freezing cells
Ability to perform protein analysis by gel electrophoresis and Western Blots
Ability to establish and maintain records and supply stocks
Skill in maintaining the lab and equipment in working condition
Ability to establish and maintain cooperative working relationships with co-workers
Demonstrated experience and skills in using the following Pipettes, Centrifuges,
Balances, Incubators, Thermal cyclers, Dry blocs, Shakers, Autoclaves, Water baths, Liquid nitrogen tanks, DNA synthesizer, PH meter, sequences
Preferred:
Ability to work with mice, setting up breeding pairs and recording births and weaning animals
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